The Inhalation Exposure Facility will provide state-of-the-art capabilities for conducting exposures of experimental animals to acrolein and butadiene. The animals will be delivered to the University of Texas Medical Branch Animal Resource Center. All shipments will be approved and overseen by personnel at the University of Louisville and the Texas Medical Branch. The animals will be exposed for varying duration to different concentrations of acrolein and butadiene, 6 h/day, 5 days/week. During exposure the general health of the animals will be monitored. After exposure the animals will be either killed for tissue and blood collection or shipped back live to the University of Louisville. In some experiments, the urine of the exposed animals will be collected and frozen samples will be shipped to Louisville. Exposure will be overseen by the Core Director and he will provide scientific input and expertise regarding experimental approaches, exposure conditions, data interpretation, and chemical and biological safety.